Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Can anyone recommend a good stable expression vector for human colorectal cancer

Started by ammahew, Dec 03 2012 10:19 AM

vectorstable expression human cell lines transfection molecular

Please log in to reply

1 reply to this topic

#1 ammahew

member

Active Members
10 posts

0
Neutral

Posted 03 December 2012 - 10:19 AM

The vector would need to be fairly cheap, show good levels of stable expression and be compatible with cell lines such as COLO205 and HCT116.

Thanks!

#2 bob1

Thelymitra pulchella

Global Moderators
4,429 posts

234
Excellent

Posted 03 December 2012 - 11:09 AM

pcDNA3 or any of that series work well. It is available from invitrogen, but many labs already have it

Back to Molecular Biology Products · Next Unread Topic →

Also tagged with one or more of these keywords: vectorstable expression, human cell lines, transfection, molecular

Protocols and Techniques Forums → Molecular Biology → Cloning cDNA construct into E.Coli. Started by Guest_rjiyer_* , 11 Jun 2013 cloning, cDNA, transfection	3 replies 109 views	bob1 Today, 02:00 PM
Protocols and Techniques Forums → Molecular Biology → Labeling and tracking RNA Started by Guest_kaveh_* , 04 Jun 2013 RNA, fluorescent, labeling and 1 more...	2 replies 142 views	doxorubicin 06 Jun 2013
Protocols and Techniques Forums → Molecular Cloning → Transient Transfection Controls (Gateway Technology) Started by Guest_greggers_* , 15 Apr 2013 Gateway, transfection, pCR3 and 1 more...	4 replies 285 views	greggers 09 May 2013
Protocols and Techniques Forums → siRNA, microRNA and RNAi → To small amount of cells used for shRNA transfection Started by Guest_Amer Mahmood_* , 15 Apr 2013 shRNA, transfection and 1 more...	1 reply 262 views	bob1 15 Apr 2013
Protocols and Techniques Forums → siRNA, microRNA and RNAi → Overexpressing miR - rescue by co-overexpressing its target Started by Guest_PBT_* , 31 Mar 2013 miRNA, microRNA, overexpression and 2 more...	1 reply 350 views	pcrman 24 May 2013