Transcription of PCR products without a primer coupled with promotor sequence
Posted 02 December 2012 - 07:03 PM
Is there a way to transcribe PCR products wihtout using a primer that has a transcription promotor. I plan to do base specific cleavage followed by MALDI TOF for strain differentiation. My PCR is a multiplex PCR with 15 primer pairs. Thus, tagging the primers is not a possibility.
Any alternatives are most welcome.
Posted 03 December 2012 - 11:47 PM
Posted 04 December 2012 - 05:40 AM
Posted 04 December 2012 - 11:24 AM
Posted 04 December 2012 - 01:00 PM