I have products from a multiplex PCR that I want to sequence using Miseq. My plan is the use Trueseq and skip the fragmentation step (my products are 270 - 450 bp) in library preparation. Will be likely to add PhiX to increase the complexity of the library before sequencing. I have never done NGS before. This is just conceptual thinking. Any suggestions/ comments are welcome.
0
PCR product sequencing using Miseq
Started by sri lankan, Dec 02 2012 06:08 PM
NGS Miseq PCR products
No replies to this topic
Also tagged with one or more of these keywords: NGS, Miseq, PCR products
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
PCR product purificationStarted by Guest_sri lankan_* , 14 Feb 2013  Purification, PCR products
|
|
|
||
Sit Back and Relax →
Conference and Workshop →
1 month left for abstracts! International Conference on Bioinformatics and CStarted by Guest_Veselin Baev_* , 25 Jul 2012 bioinformatics, conference and 2 more...
|
|
|
||
Protocols and Techniques Forums →
Research Idea, Design and Collaboration →
sample of choice for non-tumor DNAStarted by Guest_ramblingrat_* , 24 Jun 2012 NGS, non-tumor
|
|
|
||
Protocols and Techniques Forums →
Bioinformatics and Biostatistics →
Software to use for Agilent SureSelect probe designStarted by Guest_hbn_* , 06 Jun 2012 target enrichment, Agilent and 2 more...
|
|
|
