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Ethidium precipitation in CsCl Maxiprep

CsCl maxiprep trouble plasmid

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#1 BChic

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Posted 02 December 2012 - 08:34 AM

Hi Guys,

Anyone know what might cause the ethidium to precipitate to the bottom of the ultracentrifuge tube during a CsCl maxiprep?

It's happened repeatedly over the last few weeks, with several different cultures; there are small/no bands visible as well, despite having the expected size pellet during all of the preceding steps.

It's very strange and frustrating, any insight would be much appreciated.

Thanks!

#2 bob1

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Posted 02 December 2012 - 11:40 AM

Could be bound to short degraded DNA? (just an idea, don't have any real answer...)





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