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Maintainence of confluent cultures

cell culure confluent cell

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#1 MicFir

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Posted 02 December 2012 - 04:29 AM

Hi everyone,

I am looking for a good culture protocol to maintain normal primary cells in culture at a level of 70-80% conflluence at a basal, non proliferating stage for several additional days. This is in order to test the effect of cytotoxic drugs on normal, nondividing cells.

Can anyone suggest such methods?

Thanks,

Michael Firer

#2 bob1

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Posted 02 December 2012 - 11:42 AM

Look for methods relating to cell synchronization. Depending on the cells you want to use serum starvation is often a good start.




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