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Suggest best protocol for Adenosine deaminase activity

Started by srimadh, Dec 02 2012 03:04 AM

adenosine deaminase blood srimadh plasma serum

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#1 srimadh

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Posted 02 December 2012 - 03:04 AM

Hi all!

Can any one suggest me the best method to estimate the activity of adenosine deaminase in the blood.

How we can measure its activity on adenosine ... one way is to know the amount of ammonia being produced,,,

one of the articles I referred did a spec at 630 nm and quantified the ADA activity using Bertholet Method.

But, it did not work good for me ... I got all fluctuated results.. I took care of GLP ... but, I am helpless.

#2 mdfenko

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Posted 04 December 2012 - 01:01 PM

would this help?

worthington

Edited by mdfenko, 04 December 2012 - 01:02 PM.

talent does what it can
genius does what it must
i do what i get paid to do

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