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Best way to do Immunohistochemistry

histochemestry histochemistry LacZ stain

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#1 Curkobain

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Posted 28 November 2012 - 07:39 AM

Im new working in histology...im trying to do immunohistochemistry or LacZ staining but dont have good results...

i like to know the best ways to fix, dehydrated, stain, cut, etc...

#2 bob1

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Posted 28 November 2012 - 12:04 PM

These all depend on what exactly you are trying to do and what tissue(s) you are using...

#3 Curkobain

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Posted 28 November 2012 - 12:44 PM

im using mostly spleen and lymph nodes (mice) and i want to know the presence or location of a protein of interest... so i need good cuts to analyze my protein, but i have mostly "broken or fragmented" tissues...





Also tagged with one or more of these keywords: histochemestry, histochemistry, LacZ stain

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