i have isolated the microvascular endothelial cells from tumor tissue. i wanna cultivate and propagate them. but these cells can only survive for 5-7days, and then they come to senesce gradually. i use several kinds of culture medium supplemented with growth factor. still there is no use. can anyone help me?
thanks!
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#2
florencia
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Posted 19 December 2003 - 10:22 AM
Hi ,
I have developed a myoblast culture for cellular cardiomyoplasty. The cells are contaminated with fibroblast from the fascia. All the community use CD56 as a myoblast positive marker but in my flow cytometry's experiments with this antibody, my negative control with dermal fibroblast resulted positive for CD56 too.
Please help me!!
I have developed a myoblast culture for cellular cardiomyoplasty. The cells are contaminated with fibroblast from the fascia. All the community use CD56 as a myoblast positive marker but in my flow cytometry's experiments with this antibody, my negative control with dermal fibroblast resulted positive for CD56 too.
Please help me!!
#3
skinner
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Posted 13 April 2004 - 12:40 AM
metarecu, on Dec 11 2003, 06:26 AM, said:
i have isolated the microvascular endothelial cells from tumor tissue. i wanna cultivate and propagate them. but these cells can only survive for 5-7days, and then they come to senesce gradually. i use several kinds of culture medium supplemented with growth factor. still there is no use. can anyone help me?
thanks!
thanks!
#4
skinner
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Posted 13 April 2004 - 12:41 AM
metarecu, on Dec 11 2003, 06:26 AM, said:
i have isolated the microvascular endothelial cells from tumor tissue. i wanna cultivate and propagate them. but these cells can only survive for 5-7days, and then they come to senesce gradually. i use several kinds of culture medium supplemented with growth factor. still there is no use. can anyone help me?
thanks!
thanks!
