Im working with PBMC or purified lymphocytes and mi question is...
Im activating the cells with a-CD3/CD28 (1ug/ml) for 48 hrs...how much is the increase (proliferation) do i have to expect? Twice?...
Recently i have no proliferation or even less cells... In this days im going to check if they are really activated using CD69 by FACS...
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Isolated lymphocytes activation
Started by Curkobain, Nov 28 2012 06:59 AM
PBMC lymphocytes CD3/CD28
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Also tagged with one or more of these keywords: PBMC, lymphocytes, CD3/CD28
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