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cDNA, number of transcripts in bacterial vector


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#1 kajmak

kajmak

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Posted 28 November 2012 - 05:14 AM

My question is a bit basic, but ....
When making cDNA library mRNA is used. Based on cDNA synthesis kits 20-30 E. coli colonies need to be checked in order to obtain most of cDNA library. Does it mean that mRNA contains more than one gene so ds cDNA in a single vector is able to encode for more than one protein OR is E. coli able to take in more than one plasmid containing cDNA encoding for a single protein? Or is there something else I did not even consider?

Thanks on advance

kajmak




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