I am planing to differentiate human monocytes into macrophages by M-CSF. While doing flow cytometry, I want to exclude DCs from macrophages to get a pure macrophage population. I am wandering which CD markers can be used to differentiate macrophages from DC? Does anyone has any experiences of expression of CD124, CD54 on DCs? I guess, CD54 and CD124 express on macrophages, not on DC.
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CD markers that differentiate DC and macrophages
Started by Abdullah Al Tarique, Nov 27 2012 05:32 PM
Macrophage DC monocytes CD124 CD54
1 reply to this topic
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Curtis
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Posted 27 November 2012 - 06:48 PM
Here, check this out first:
http://www.pathology.../cdmarkers.html
CD54 is very broad according to this page, but CD124 has not been found on DCs.
Check CD205 and CD206 also. Apparently they are unique to DCs
http://www.pathology.../cdmarkers.html
CD54 is very broad according to this page, but CD124 has not been found on DCs.
Check CD205 and CD206 also. Apparently they are unique to DCs
