I am facing a problem regarding qPCR quantitation, and I hope, that someone in this forum might help me.
Currently I am working in a research project in which we perform a gene expression study of human monocytes. There is one group of critically ill patients, and another group which serves as a control group. However I want to perform both quantitation via deltadelta CT method in case PCR efficiencies are comparablem, but also quantitation via relative standard curves.
Now my question is: Which material is suitable for relative standard curve quantitation. I am not interested in absolute quantitation because I am comparing two groups together, but I am interested in how much mRNA content is in the cells independently regarding pcr efficiency.
Would it be appropriate to use THP-1 cells for reference rna?
Edited by AlexanderA, 26 November 2012 - 01:55 PM.