SDS PAGE gels
Posted 26 November 2012 - 12:09 PM
I have been working with proteins with molecular vweight of 150 Kda and 250 Kda.But I am unable to see the proper fragment at the exact size as it gets stuck in the Stacking gel(4%).I am using Tris-Glycine gels of 8% resolving and 4%stacking portions and using overnight transfer at 25V with PVDF membrane at 4 degree temperature.Please let me know how to resolve this problem so that i can see a proper fragment of my protein.
Posted 26 November 2012 - 02:27 PM
Posted 22 January 2013 - 08:25 PM
Posted 30 January 2013 - 10:07 AM
I have problems with my samples (which are flushing fluids from mare´s uterus) , i use 2D-gel electrphoresis but the results were disappointed .Really,i dont know what should i do?
Anybody can help me please?
Posted 30 January 2013 - 11:37 AM