in vivo naked-mRNA deliverymRNA delivery
Posted 26 November 2012 - 01:55 AM
this might seem a rather-not-related subject as this is a miRNA forum, but I was wondering if any body has any kind of experince with in vivo naked-mRNA delivery, possibly using simple soltins like ringer's or PBS.
Posted 28 November 2012 - 10:25 PM
This recent paper should have cited the original paper describing this approach
Posted 29 November 2012 - 11:07 PM
Posted 13 December 2012 - 01:29 PM
what organ are you trying to target in vivo?
Some researchers have been successful with siRNA infusion using minipump in the brain (I.e Thacker et al.)
Posted 12 January 2013 - 11:16 PM
change of planes! if I were to transfect cell lines with nacked- invitro transcribed- mrna, what would be the best and cheapest method? could I use DNA transfecting reagents? dose anyone have any experience with lipofectamin ltx( plus).
thank you all