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in vivo naked-mRNA delivery

mRNA delivery

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4 replies to this topic

#1 mahrak

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Posted 26 November 2012 - 01:55 AM

hi all
this might seem a rather-not-related subject as this is a miRNA forum, but I was wondering if any body has any kind of experince with in vivo naked-mRNA delivery, possibly using simple soltins like ringer's or PBS.

#2 pcrman

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Posted 28 November 2012 - 10:25 PM

Have you read the papers on delivering mRNA of stem cell factors to reprogram somatic cells into iPS cells? I believe the mRNA has to be modified to make it stable.

This recent paper should have cited the original paper describing this approach

http://www.nature.co.../srep00657.html

#3 mahrak

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Posted 29 November 2012 - 11:07 PM

tnx pcr man. stabilizing RNA's backbon is an excellent approache and has been utilizied in a lot of rna studies, however naked not-inhanced rna has proven effective too. I couldn't find anything on the formulation though, other than " ringer solution" or "PBS"
http://www.ncbi.nlm.nih.gov/pubmed/20961459

#4 Xavier2

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Posted 13 December 2012 - 01:29 PM

Hello,

what organ are you trying to target in vivo?
Some researchers have been successful with siRNA infusion using minipump in the brain (I.e Thacker et al.)
Xavier

#5 mahrak

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Posted 12 January 2013 - 11:16 PM

hello again

change of planes! if I were to transfect cell lines with nacked- invitro transcribed- mrna, what would be the best and cheapest method? could I use DNA transfecting reagents? dose anyone have any experience with lipofectamin ltx( plus).

thank you all




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