4 replies to this topic

[mahrak]

hi all
this might seem a rather-not-related subject as this is a miRNA forum, but I was wondering if any body has any kind of experince with in vivo naked-mRNA delivery, possibly using simple soltins like ringer's or PBS.

[pcrman]

Have you read the papers on delivering mRNA of stem cell factors to reprogram somatic cells into iPS cells? I believe the mRNA has to be modified to make it stable.  

This recent paper should have cited the original paper describing this approach

http://www.nature.co.../srep00657.html

[mahrak]

tnx pcr man. stabilizing RNA's backbon is an excellent approache and has been utilizied in a lot of rna studies, however naked not-inhanced  rna has proven effective too. I couldn't find anything on the formulation though, other than " ringer solution" or "PBS"
http://www.ncbi.nlm....pubmed/20961459

[Xavier2]

Hello,

what organ are you trying to target in vivo?
Some researchers have been successful with siRNA infusion using minipump in the brain (I.e Thacker et al.)
Xavier

[mahrak]

hello again

change of planes! if I were to transfect cell lines with nacked- invitro transcribed- mrna, what would be the best and cheapest method? could I use DNA transfecting reagents? dose anyone have any experience with lipofectamin ltx( plus).

thank you all