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# Can medium containing starch be autoclaved?

Starch autoclave medium

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### #31 SunsetSatine

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Posted 12 December 2012 - 01:51 PM

Ah! I see. Darn. 9g/100 mL puts my concentration at 9%. Per my calculations, I need to prepare 20%. So, the calculations that I did above need to be adjusted from 20% to 9% correct?

### #32 SunsetSatine

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Posted 12 December 2012 - 02:00 PM

I went ahead and add 50 mL of DI water to the solution to dilute it down, and it all dissolved. Does this mean that my solution is still 10% concentration? Or given the solubility, is it actually at 9% concentration?

### #33 pito

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Posted 13 December 2012 - 08:34 AM

SunsetSatine, on 12 December 2012 - 02:00 PM, said:

I went ahead and add 50 mL of DI water to the solution to dilute it down, and it all dissolved. Does this mean that my solution is still 10% concentration? Or given the solubility, is it actually at 9% concentration?

10grams in 100ml or?
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### #34 SunsetSatine

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Posted 13 December 2012 - 09:15 AM

Final volume was 100 mL

### #35 pito

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Posted 13 December 2012 - 09:27 AM

SunsetSatine, on 13 December 2012 - 09:15 AM, said:

Final volume was 100 mL

Wel then you have 10grams in 100Mml...

10% solution
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### #36 hobglobin

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Posted 13 December 2012 - 09:47 AM

pito, on 13 December 2012 - 09:27 AM, said:

SunsetSatine, on 13 December 2012 - 09:15 AM, said:

Final volume was 100 mL

Wel then you have 10grams in 100Mml...

10% solution
But not if only 9g are soluble in 100 mL....at room temperature there should be a precipitate visible of the remaining gram.

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### #37 pito

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Posted 13 December 2012 - 09:50 AM

hobglobin, on 13 December 2012 - 09:47 AM, said:

pito, on 13 December 2012 - 09:27 AM, said:

SunsetSatine, on 13 December 2012 - 09:15 AM, said:

Final volume was 100 mL

Wel then you have 10grams in 100Mml...

10% solution
But not if only 9g are soluble in 100 mL....at room temperature there should be a precipitate visible of the remaining gram.

I am assuming he was able to dissolve it completely.

But indeed, you are right, is there is precipitation then its not 10% rather 9%
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.