I am studying different spliced isoforms from a particular gene by RT-qPCR. I get amplification from a particular intron. How can I know whether the amplification is coming from pre mRNA? Can one 'clean' somehow the mRNA from the pre mRNA?
Thank you.
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pre mRNA after total mRNA isolation?
Started by Niwrad, Nov 21 2012 06:52 AM
mRNA precursor mRNA splicing mRNA processing qPCR
2 replies to this topic
#2
mdfenko
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Posted 21 November 2012 - 07:40 AM
pre-mrna is processed so quickly that it is highly unlikely to be present in sufficient quantities to interfere.
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genius does what it must
i do what i get paid to do
#3
Trof
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Posted 19 December 2012 - 08:28 AM
It can also come from the traces of DNA, that is far more likely. You can use DNAse to clen your sample.
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