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pre mRNA after total mRNA isolation?

mRNA precursor mRNA splicing mRNA processing qPCR

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2 replies to this topic

#1 Niwrad

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Posted 21 November 2012 - 06:52 AM

I am studying different spliced isoforms from a particular gene by RT-qPCR. I get amplification from a particular intron. How can I know whether the amplification is coming from pre mRNA? Can one 'clean' somehow the mRNA from the pre mRNA?

Thank you.

#2 mdfenko

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Posted 21 November 2012 - 07:40 AM

pre-mrna is processed so quickly that it is highly unlikely to be present in sufficient quantities to interfere.
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#3 Trof

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Posted 19 December 2012 - 08:28 AM

It can also come from the traces of DNA, that is far more likely. You can use DNAse to clen your sample.

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Also tagged with one or more of these keywords: mRNA precursor, mRNA splicing, mRNA processing, qPCR

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