I'm new here and interested in DNA methylation regulation. I use the EZ methylation kit to do treatment of bisufite, and then I stored my treated DNA at -80oC. Do someone have an idea that whether the proportion of methylation in bisulfited DNA will change with longterm conservation or frequent temperature changes (thawing for experiment)??? And have you tested it ? (I wonder the strand which contains uracil, it maybe unstable, so it's easy to break?)
Thans a lot !
Edited by Leon Liu, 21 November 2012 - 05:51 AM.