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Serum inactivation:

serum antibodies

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2 replies to this topic

#1 @bhijit

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Posted 20 November 2012 - 04:40 PM

I have read many studies genrerally inactivate the serum used in assays - inactivation at 56 degree for 30 min.
1. Why this is done ?
2. Is this inactivation done only for specific experiments like - Invitro inhibition, ELISA etc ?

Thank you for your time.

#2 science noob

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Posted 20 November 2012 - 07:37 PM

http://atcc.custhelp...ctivating-serum

#3 Nimal Raveendran

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Posted 20 November 2012 - 08:59 PM

Heat inactivation was 1c deemed necessary 2 destroy heat labile components,such as complements.

In addition 2 complement inactivation, heat inactivation was believed 2 inactivate adventitious microbial contaminants such as mycoplasmas...

U can find drawbacks of heat inactivation here:

heat inactivation increases the formation of precipitates (which are frequently mistaken for a microbial contaminant) & decreases d growth promotion capacity of d serum

TY
:)

https://fscimage.fishersci.com/cmsassets/downloads/segment/Scientific/pdf/Cell_Culture/Application_Notes/heat_inactivation.pdf





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