Posted 19 November 2012 - 05:57 PM
Growing bacteria for plasmid minipreps usually requires a 12-16hr incubation. Longer incubation can cause cell lysis and loss of plasmid. Too short of incubation means less growth and less plasmid. Sometimes I don't have time to miniprep when the bacteria are ready, especially when I have 20 samples or so. Is there a good stopping point? I was wondering if I could remove my culture at 12-16 hours, pellet the cells, aspirate the media and refrigerate or freeze the pellet until I have time to do the miniprep? I thought this would be ok as long as the media is completely removed otherwise the cells would lyse during the freeze-thaw and release plasmid which would be lost during aspirating.