Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Checking pH of RNase Free Solutions

RNase Free RNA RNA Denaturing Gel RNA Extraction

  • Please log in to reply
1 reply to this topic

#1 Epigeneticist

Epigeneticist

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 97 posts
5
Neutral

Posted 19 November 2012 - 05:17 PM

Trying to prepare reagents for Denaturing RNA gel. Is it possible for the pH probe or storage buffer to be contaminated with RNase? Can the probe be rinsed with DEPC treated water or should I take an aliquot of the solution and use pH paper to check the pH. I was wondering how I can check the pH of RNase free solution without contaminating it with RNase?

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,726 posts
399
Excellent

Posted 19 November 2012 - 07:38 PM

Yes, it is possible for the probe to be contaminated with RNase, and rinsing it with DEPC treated water will not get rid of the RNases as the DEPC is by this time CO2 and H2O. You need to take an aliquot and measure the pH in that with the probe if possible, pH paper isn't all that accurate; then throw the aliquot out, ...repeat until desired pH is reached.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.