U87MG cells not growing well
Posted 18 November 2012 - 10:37 PM
Posted 19 November 2012 - 12:05 AM
If the former, then throw them out and get up a fresh tube. You should be doing this every 10 passages or so anyway, as the growth rate and behaviour of cells changes with increasing culture time.
If the latter; different cell lines have different growth rates, some like Hela will grow extremely fast with doubling times of about 18-20 hours, while others might only double every 2-3 days.
Posted 20 November 2012 - 04:24 AM
- add more cells at seeding
- use nuclear staining and check fluorescence to investigate the cells viability.
Other Suggestion- wash(fast) with solution of antibiotic.
Don't forget to be good mood, smile all the time, have nice music as ambient that may help.