Concentrating low volume of cells
Posted 17 November 2012 - 08:25 PM
Posted 17 November 2012 - 08:49 PM
I would try gently pouring it off, trying not to disrupt the cell pellet, and if that means leaving a little behind, that's ok.
Posted 17 November 2012 - 09:14 PM
Edited by Engi, 17 November 2012 - 09:15 PM.
Posted 17 November 2012 - 09:21 PM
Also, I have found that centrifuge speeds that I use for my big tubes (15 and 50ml) in the larger benchtop centrifuges pellet my cells MUCH better than equivalent rcf values in a microfuge. I don't know why this is (350xg for both).
I get around it by increasing the speed for the microfuge- but by this stage my cells are usually fixed and so I don't have to worry about viability.
Hopefully someone else has some tips for you
- Denny likes this
Posted 18 November 2012 - 07:22 AM
As for cfg speed, depending on the cell line, I pellet mine at 800 - 950 rpm without losing viability. Will your cells tolerate a higher rpm/g's?
Posted 18 November 2012 - 11:30 AM
Posted 18 November 2012 - 11:41 AM
The larger tubes thing will help some and extended pelleting time can help, but really - don't spin at higher RCF!
Posted 18 November 2012 - 11:58 AM
Thanks for the catch Bob I re-read the original post and see that Engi was spinning cells at 750xg not RPM, my very very bad
Edited by Denny, 18 November 2012 - 12:03 PM.
Posted 18 November 2012 - 01:42 PM