Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

DNA EtOH/Sodium Acetate precip over the weekend...still good?


  • Please log in to reply
2 replies to this topic

#1 Denny

Denny

    Denny Is As Denny Does

  • Active Members
  • PipPipPipPipPip
  • 78 posts
4
Neutral

Posted 17 November 2012 - 06:46 PM

Hey Gang,
Not my preferred method, but long week, long hours, had to leave my plasmid DNA in EtOH and sodium acetate at -20 C for the weekend. I haven't left it this long before, does anyone have thoughts or experience with this. I'm worried about the DNA being any good. Seems like it should be fine if it's precipitated and just hanging out in the -20C, but having never tested it, it's bugging me tonight. I know, get a life!

#2 leelee

leelee

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 652 posts
53
Excellent

Posted 17 November 2012 - 08:51 PM

It will be completely fine. It is actually a good way to store DNA long term.

#3 Denny

Denny

    Denny Is As Denny Does

  • Active Members
  • PipPipPipPipPip
  • 78 posts
4
Neutral

Posted 17 November 2012 - 08:55 PM

Awesome, thank you!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.