Western blotting quantification and normalisation
Posted 08 December 2003 - 02:18 AM
I would like to quantify signals from western blotting.
1. Should I repeat a sample (positive control) between all my gels?
2. What's the right way to normalise my data? Should I normalise in relationship with beta-actin (house-keeping) and also with my positive control?
Thanks for all inputs
Posted 11 December 2003 - 08:53 AM
Posted 18 December 2003 - 01:54 PM