I've used 4M Guanidine Hydro Chloride (GuHCl) for Plant RNA Viroid extraction with 25mM EDTA, 0.2M Sodium Acetate (NaAc) (pH 5), 2.5% PVP 40. Could you please tell me ;
How RNA get separated from DNA when using GuHCl?
What is the purpose of adding NaAc in Nucleic Acid Extraction?
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What happens to DNA in the presence of a chaotropic salt?
Started by Akila Wijerathna Yapa, Nov 17 2012 04:38 AM
RNA Extraction Viroids NaAc GuHCl
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#1
Akila Wijerathna Yapa
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Posted 17 November 2012 - 04:38 AM
#2
memari
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Posted 17 November 2012 - 08:39 AM
What is the purpose of adding NaAc in Nucleic Acid Extraction?
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Babak Memari
Babak Memari
#3
bob1
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Posted 17 November 2012 - 12:27 PM
Guanidine is a chaotrope, so it essentially denatures the proteins so that they won't stick to the DNA.
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Akila Wijerathna Yapa
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Posted 18 November 2012 - 11:18 PM
Thank you very much memari & bob1
