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What happens to DNA in the presence of a chaotropic salt?

RNA Extraction Viroids NaAc GuHCl

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#1 Akila Wijerathna Yapa

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Posted 17 November 2012 - 04:38 AM

I've used 4M Guanidine Hydro Chloride (GuHCl) for Plant RNA Viroid extraction with 25mM EDTA, 0.2M Sodium Acetate (NaAc) (pH 5), 2.5% PVP 40. Could you please tell me ;

How RNA get separated from DNA when using GuHCl?

What is the purpose of adding NaAc in Nucleic Acid Extraction?

#2 memari

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Posted 17 November 2012 - 08:39 AM

What is the purpose of adding NaAc in Nucleic Acid Extraction?
http://www.protocol-...posts/3846.html
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Babak Memari

#3 bob1

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Posted 17 November 2012 - 12:27 PM

Guanidine is a chaotrope, so it essentially denatures the proteins so that they won't stick to the DNA.

#4 Akila Wijerathna Yapa

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Posted 18 November 2012 - 11:18 PM

Thank you very much memari & bob1





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