I've used 4M Guanidine Hydro Chloride (GuHCl) for Plant RNA Viroid extraction with 25mM EDTA, 0.2M Sodium Acetate (NaAc) (pH 5), 2.5% PVP 40. Could you please tell me ;
How RNA get separated from DNA when using GuHCl?
What is the purpose of adding NaAc in Nucleic Acid Extraction?
0
What happens to DNA in the presence of a chaotropic salt?
Started by Akila Wijerathna Yapa, Nov 17 2012 04:38 AM
RNA Extraction Viroids NaAc GuHCl
3 replies to this topic
#1
Akila Wijerathna Yapa
-
- Active Members
-
- 13 posts
member
0
Neutral
Neutral
Posted 17 November 2012 - 04:38 AM
#2
memari
-
- Active Members
-
- 99 posts
Enthusiast
6
Neutral
Neutral
Posted 17 November 2012 - 08:39 AM
What is the purpose of adding NaAc in Nucleic Acid Extraction?
http://www.protocol-...posts/3846.html
http://www.protocol-...posts/3846.html
-----
Babak Memari
Babak Memari
#3
bob1
-
- Global Moderators
-
- 4,429 posts
Thelymitra pulchella
234
Excellent
Excellent
Posted 17 November 2012 - 12:27 PM
Guanidine is a chaotrope, so it essentially denatures the proteins so that they won't stick to the DNA.
#4
Akila Wijerathna Yapa
-
- Active Members
-
- 13 posts
member
0
Neutral
Neutral
Posted 18 November 2012 - 11:18 PM
Thank you very much memari & bob1
