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Mass-spectrometry of membrane proteins

Started by Andrey, Nov 16 2012 02:43 PM

membrane protein MALDI trypsin

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#1 Andrey

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Posted 16 November 2012 - 02:43 PM

Can somebody point me to a an easy protocol to prepare membrane protein for MALDI analysis? It is inserted into lipid liposomes and I would like to do tryptic digestion. .The problem is that trypsin fails to reach hydrophobic regions.

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#2 Schevchen

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Posted 10 December 2012 - 08:39 AM

maybe you can use chymotrypsin

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