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qRT-PCR data analysis


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#1 sayeeys

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Posted 16 November 2012 - 11:26 AM

I got the ct and 2^ddct values. I am confused as to how to calculate the fold change and p-values using Q-Gene software. Also, any guidance to create graphs or table is appreciated. I am comparing Wild type with the mutant to check the expression (gene regulation). I also want to know how to set the p-value?

#2 Trof

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Posted 16 November 2012 - 01:12 PM

Making several topics about the same problem doesn't really help (though changing the title to something else than "Help" may sometimes substantially).
Did you tried to find a manual or read the original paper/presentation?

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#3 sayeeys

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Posted 17 November 2012 - 12:47 PM

Yes, I did. As this my first time it is a little confusing. I am trying to work on this, but any suggestions will be helpful.

P.S. The reason to post the same problem is because I was unable to change the title for my earlier post. Well, the answer is in your response itself. "(though changing the title to something else than "Help" may sometimes substantially)."




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