Western blotting quantification and normalisation
Posted 08 December 2003 - 12:59 AM
I would like to quantify signals from western blotting.
1. Should I repeat a sample (positive control) between all my gels?
2. What's the right way to normalise my data? Should I normalise in relationship with beta-actin (house-keeping) and also with my positive control?
Thanks for all inputs
Posted 09 January 2004 - 02:25 AM
Posted 01 March 2004 - 07:48 PM
Try cdk2 detection.... very strong and usually consistent.
Posted 14 March 2004 - 07:16 AM
GAPDH could also be an internal standard
but I wonder know,
the steps in normalizing my bands across 2 comparing samples using the internal standard...
since protein loading doesn't perfectly the same in each time...so the band of internal control could vary slightly
thanks very much for any opinion