Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Peprotech IP-10 ELISA

Peprotech IP-10 ELISA

  • Please log in to reply
No replies to this topic

#1 Chikopiko



  • Active Members
  • Pip
  • 5 posts

Posted 16 November 2012 - 09:24 AM

I am using the Peprotech kit for IP-10 Elisa. I was using the standards provided by the kit and did serial dilutions, the last point of 0 pg/ml was used as diluent alone. However, when I was measuring my results I realized that some of the tested cells gave me lower O.D values than the O.D of the 0 pg/ml Standard. Thus when I calculated the IP-10 values in my experiment some of the tested wells had a negative value in terms of pg/ml. Has anyone experienced suce a thing? Is there any way to sove this issue? Also It took a while (about 30 min) until the color developed to give O.D values of 0.9 for the 1000 pg/ml standard.

Thank you!!!!

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.