Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

What is the most common and updated database?

  • Please log in to reply
2 replies to this topic

#1 Nicole Wa

Nicole Wa


  • Members
  • Pip
  • 4 posts

Posted 16 November 2012 - 02:20 AM

Dear all,
I am trying to blasting the collected sequences of transcripts. But I am confusing of what database is better choice. Because the information in different database is shown with different result (>@<). Besides, people said the genomic databases are modified often, then how can I know which sequence in database is last update or is more confident.

Edited to fix your text size - Bob.

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,668 posts

Posted 16 November 2012 - 11:21 PM

You probably need to look for the Refseq - which is the curated form and the "definitive" version. You can also see the date of upload on the entry, but I don't know how to find the most recent.

#3 Trof


    Brain on a stick

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,384 posts

Posted 17 November 2012 - 07:56 AM

As far as I know human sequences for example were all merged recently into one reference sequence for each chromosome, so there is no more multiple contigs. But since more than thousand human genomes have been completely sequence by now, the question of what sequence is actually reference wil probably appear in future, but that would be really mostly a matter of SNPs only.

As bob1 said, when blasting human or mouse select Genomic+transcript database, which should contain reference sequences only, and for any other choose refseq database, maybe limit it by a species you work with in the field below (if you don't want to blast across species of course).

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.