2 replies to this topic

[Nicole Wa]

Dear all,
I am trying to blasting the collected sequences of transcripts. But I am confusing of what database is better choice. Because the information in different database is shown with different result (>@<). Besides, people said the genomic databases are modified often, then how can I know which sequence in database is last update or is more confident.

Edited to fix your text size - Bob.

[bob1]

You probably need to look for the Refseq - which is the curated form and the "definitive" version.  You can also see the date of upload on the entry, but I don't know how to find the most recent.

[Trof]

As far as I know human sequences for example were all merged recently into one reference sequence for each chromosome, so there is no more multiple contigs. But since more than thousand  human genomes have been completely sequence by now, the question of what sequence is actually reference wil probably appear in future, but that would be really mostly a matter of SNPs only.

As bob1 said, when blasting human or mouse select Genomic+transcript database, which should contain reference sequences only, and for any other choose refseq database, maybe limit it by a species you work with in the field below (if you don't want to blast across species of course).