Posted 07 December 2003 - 10:09 AM
Posted 07 December 2003 - 09:49 PM
I don't know what you exactly meant by cycling conditions: Ta or cycles? If there is a difference in Ta, you can select a Ta half way between those two Tas, since Ta usually works in a wide range. Or you can redesign a pair of primers with Ta close to the other pair. If there is a difference in cycling number, you can adjust the amount of primers added to your PCR reaction. For example, if you amplify the two genes using 30 cycles, and only one show product, then you should reduce the amount of primers for that gene and increase cycles.
You need optimization (means repeat and repeat) before you can get a desired picture. Also there are software which can design multiplex primer (search google).
Hope it's useful.
Edited by labrat, 07 December 2003 - 09:51 PM.