17 kDa ideal transfer voltage - PVDF
Posted 15 November 2012 - 03:17 PM
Wondering what people would use ideally to transfer a 17kDa protein as efficiently as possibly onto PVDF? I have been doing 1.5 hours at 75V (constant voltage) but am slightly losing the low range. Open for some ideas here. Thanks!
Posted 15 November 2012 - 04:09 PM
Posted 15 November 2012 - 04:30 PM
Posted 15 November 2012 - 05:10 PM
Posted 16 November 2012 - 11:04 AM
you can determine if you are blowing your protein through the membrane by using a backup membrane.
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