Preparing a conditioned medium from cancer cells
Posted 15 November 2012 - 02:25 PM
I am working with HUVECs and the effects of VEGF on its growth and metabolism. I plan to use a conditioned medium from a cancer cell line. But I would like to know, if, in order to have enough VEGF or any other factor to activate angiogenesis in this medium is it sufficient that the conditioned medium be taken from growing the cancer cells in a normal manner? Is it not required to grow the cancer cells in a stressed or special (tumour micro) environment when these factors will be released actually, as in the real situation in human body?
Posted 15 November 2012 - 05:17 PM
I don't know the answer to your actual question, other than to say that many cancer cell lines are happily secreting a bunch of different growth factors all the time, so it may not be necessary to stress them.
Posted 16 November 2012 - 06:41 AM
I do use a recombinant VEGF directly, but am also interested in random factors, as in the conditioned medium.
Posted 16 November 2012 - 07:47 PM