I am havign trouble getting my h-tert rpe1 cells to form primary cilia. I am using sigma anti-acetylated tubulin mouse monoclonal antibody. I serum starve (0.25% FBS) them for 48 hours the day after plating them. I have tried multiple plating concentrations and stained everywhere from 80-100% confluency and still no luck. I am getting maybe 10 clear primary cilia for every 500 cells. Can anyone help me?
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