I am havign trouble getting my h-tert rpe1 cells to form primary cilia. I am using sigma anti-acetylated tubulin mouse monoclonal antibody. I serum starve (0.25% FBS) them for 48 hours the day after plating them. I have tried multiple plating concentrations and stained everywhere from 80-100% confluency and still no luck. I am getting maybe 10 clear primary cilia for every 500 cells. Can anyone help me?
Submit your paper to J Biol Methods today!
No replies to this topic