Sodium Phosphate Buffer
Posted 15 November 2012 - 03:12 AM
I have an issue regarding production of sodium phosphate buffer.
I have been asked to make 2x sodium phosphate buffer pH8.0. I have found protocols which combine 1M monobasic and 1M dibasic reagents and adjust to pH using either or which I have made.
My issue is how do I know what 2x is? and or, what would the combination of the two 1M solutions produce in 'X'. Im hoping to make a stock like PBS comes in @ 10x and then dilute down from there.
Hope this makes sense
Posted 15 November 2012 - 12:01 PM
Posted 15 November 2012 - 04:04 PM
Posted 15 November 2012 - 05:12 PM
Posted 16 November 2012 - 09:01 AM
genius does what it must
i do what i get paid to do