Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

phage lysate purification


  • Please log in to reply
2 replies to this topic

#1 favy

favy

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 14 November 2012 - 06:07 AM

dear researchers.
i need technical help though the situation for me is lil bit weird.i have used a phage to lyse a bacterium.after getting the phage lysate.i have to separate out my bacterial lysate from the phage.guys i dont have to purify the phage .i need to recover the lysed cellular products from the phage with minimum damage to the proteins in the lysate .ofcourse we do have centrifugation and PEG to purify or separate out the phage but i cant use them coz my bacterial proteins will be altered.are there any filters that could be used to separate out the lysed bacterial products from the phage or any other procedure.beside proteins to be recovered in the lysate fall below 150Kda.

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,800 posts
407
Excellent

Posted 14 November 2012 - 01:13 PM

You can get molecular weight cut-off spin filters from a number of places. Amicon is probably the best known of the manufacturers

#3 favy

favy

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 15 November 2012 - 06:13 AM

thanx for the suggestion.i will try out




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.