Quantify gDNA in buccal swab
Posted 13 November 2012 - 11:52 AM
I'm trying to perform PCRs direct on buccal swab material (no lysis, just buccal swab in water then vortex). I fear I'm getting very low DNA yield but what is the quickest way to check DNA quantity? Agarose gel would just be a smear,right?
OD260 would be meaningless with all cell debris RNA etc.?
Sorry if this has been asked before, I've been searching for half an hour and could find anything complete
Posted 13 November 2012 - 11:59 AM
Posted 29 November 2012 - 02:29 PM
- It yields 5-10 ug of DNA from a dried specimen swab, instead of 200-500 ng.
- It improves detection sensitivity.
- It's nontoxic.
- Its lysate may be used for PCR without further DNA purification.
- Dried specimen swabs can be shipped and stored long-term at room temperature.