Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Studying epigenetics of paternal or maternal allele

epigenetics imprinting method

  • Please log in to reply
4 replies to this topic

#1 GoldEpi

GoldEpi

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 12 November 2012 - 01:42 PM

Hi everybody,

I have been wondering and searching but haven't found a method that enables distinguishing the paternal and maternal allele with all the modifications and proteins bound to DNA. This would be extremely helpful when studying imprinted genes and their regulatory proteins and modifications - now bisulfite seq and ChIP only give a mixed result.

Separating the alleles could be possible at the chromosome level, but how? Any ideas or comments? I have heard about some technique that uses mitosis as a starting point but the regulatory mechanisms cannot be studied with the same strategy. I have thought that artificial triplex structures in the area of many maternal/paternal SNPs could serve as a starting point for chromosome capture but applying this technique might be impossible.

Don't hesitate to share your ideas or comments, I'm looking forward to them!

Edited by GoldEpi, 12 November 2012 - 01:46 PM.


#2 Trof

Trof

    Brain on a stick

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,149 posts
101
Excellent

Posted 12 November 2012 - 02:37 PM

We have a model, where we transfered single specific human chromosome into MEL cell line. But I'm not sure whether the modifications will be conserved along the cell divisions.

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon


#3 GoldEpi

GoldEpi

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 13 November 2012 - 12:49 AM

Thanks for the reply. Indeed, artificial biochemical microenvironments and cell divisions will affect the combination of regulatory factors. This cell line would at least help indentifying paternal/maternal SNPs. Have you published a paper about making this cell line?

#4 Trof

Trof

    Brain on a stick

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,149 posts
101
Excellent

Posted 13 November 2012 - 09:27 AM

We used similar approach as descirbed in this original paper, but used patients EBV-transformed lymphocytes instead of fetal cells.


Papayannopoulou T, Brice M, Stamatoyannopoulos G. Analysis of human hemoglobin switching in MEL x human fetal erythroid cell hybrids. Cell. 1986 Aug 1;46(3):469-76.

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon


#5 chabraha

chabraha

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 137 posts
2
Neutral

Posted 15 November 2012 - 02:44 PM

http://www.ncbi.nlm....pubmed/22948768
Treasure Chest Wizardry





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.