hi! i have a gene of interest cloned in the vector pGEM3Z. I would like to know whether it is possible to express the protein in this vector itself ( since it has a promoter region and termination region). If yes what is the suitable strain for expression. can i use BL21 DE3. Do i have to induce expression by adding IPTG for this strain. my gene is under the SP6 promoter.
Thank you in advance for your help.
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cecile
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Posted 04 August 2002 - 05:07 AM
Usually Bl21 are good for expression of recombinant bacteria.
Are you sure your promoter (Sp6) is a procaryote promoter ? I may do a mistake but I think that sp6 promoter is used for in vitro translation in eucaryote cells.
Usually to expressed protein in bacteria we use plasmid such as PGEX (look in clonetech catalog).
Are you sure your promoter (Sp6) is a procaryote promoter ? I may do a mistake but I think that sp6 promoter is used for in vitro translation in eucaryote cells.
Usually to expressed protein in bacteria we use plasmid such as PGEX (look in clonetech catalog).
