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help for blood collection and centrifugation for ELISA

Started by Frankie, Nov 12 2012 08:51 AM

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#1 Frankie

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Posted 12 November 2012 - 08:51 AM

Hello!
Is it still okay to centrifuge and collect plasma or serum from frozen rat whole blood samples?
I have frozen rat whole blood samples immediately after collection and stored in -80. I was not able to centrifuge them to collect serum and plasma for ELISA (BDNF or corticosterone) immediately. Is there any protocol for this?
Thanks !!!

#2 bob1

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Posted 12 November 2012 - 11:16 AM

The blood cells will have lysed so you won't be able to efficiently remove them.
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