Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

help for blood collection and centrifugation for ELISA


  • Please log in to reply
1 reply to this topic

#1 Frankie

Frankie

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 12 November 2012 - 08:51 AM

Hello!
Is it still okay to centrifuge and collect plasma or serum from frozen rat whole blood samples?
I have frozen rat whole blood samples immediately after collection and stored in -80. I was not able to centrifuge them to collect serum and plasma for ELISA (BDNF or corticosterone) immediately. Is there any protocol for this?
Thanks !!!

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,721 posts
399
Excellent

Posted 12 November 2012 - 11:16 AM

The blood cells will have lysed so you won't be able to efficiently remove them.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.