Edited by science noob, 12 November 2012 - 03:17 AM.
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#1
science noob
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Posted 12 November 2012 - 03:16 AM
Accidentally forgot to keep my aliquot of plasmid DNA back into the freezer and was left on my lab bench for a few days (at RT). What are the ramifications of this action? Plasmid will be intended for transfection.
#2
Ameya P
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Rervm Cognoscere Cavsas
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Posted 12 November 2012 - 04:33 AM
If it was in TE buffer and 20-25 oC for few days, it should be fine.
Go ahead, it should work.
Go ahead, it should work.
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#3
bob1
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Posted 12 November 2012 - 11:24 AM
You can always re-transform it, or grow up some more and do another prep...
#4
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Posted 12 November 2012 - 02:37 PM
Ameya P, on 12 November 2012 - 04:33 AM, said:
If it was in TE buffer and 20-25 oC for few days, it should be fine.
Go ahead, it should work.
Go ahead, it should work.
What about 20-25oC for a few days in water?
#5
Trof
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Posted 12 November 2012 - 02:42 PM
I once accidentally forgot plasmids on the bench for more than a month. After that I had to put some on Whatman paper and send overseas by mail. They sucessfully transformed and never noticed anything 
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