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Plasmid DNA at RT for a few days


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#1 science noob

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Posted 12 November 2012 - 03:16 AM

Accidentally forgot to keep my aliquot of plasmid DNA back into the freezer and was left on my lab bench for a few days (at RT). What are the ramifications of this action? Plasmid will be intended for transfection.

Edited by science noob, 12 November 2012 - 03:17 AM.


#2 Ameya P

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Posted 12 November 2012 - 04:33 AM

If it was in TE buffer and 20-25 oC for few days, it should be fine.

Go ahead, it should work.

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#3 bob1

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Posted 12 November 2012 - 11:24 AM

You can always re-transform it, or grow up some more and do another prep...

#4 science noob

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Posted 12 November 2012 - 02:37 PM

If it was in TE buffer and 20-25 oC for few days, it should be fine.

Go ahead, it should work.


What about 20-25oC for a few days in water?

#5 Trof

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Posted 12 November 2012 - 02:42 PM

I once accidentally forgot plasmids on the bench for more than a month. After that I had to put some on Whatman paper and send overseas by mail. They sucessfully transformed and never noticed anything ;)

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