What is the yield of BMDC from 1 mouse BM?
If I get 3X10^7 BM cells from 1 mouse and how many DC I can get from these cells after 5-7 day culture (IL-4 and GMCSF, 10ng/ml)?
Thanks!!
Edited by hj_chris, 12 November 2012 - 12:56 AM.
0
2 replies to this topic
#1
hj_chris
-
- Members
-
- 4 posts
member
0
Neutral
Neutral
Posted 11 November 2012 - 07:26 PM
Dear,
What is the yield of BMDC from 1 mouse BM?
If I get 3X10^7 BM cells from 1 mouse and how many DC I can get from these cells after 5-7 day culture (IL-4 and GMCSF, 10ng/ml)?
Thanks!!
What is the yield of BMDC from 1 mouse BM?
If I get 3X10^7 BM cells from 1 mouse and how many DC I can get from these cells after 5-7 day culture (IL-4 and GMCSF, 10ng/ml)?
Thanks!!
#2
hj_chris
-
- Members
-
- 4 posts
member
0
Neutral
Neutral
Posted 12 November 2012 - 12:55 AM
Anyone know?
I can only get 6 million BMDC from 1 mouse, and only 1 million BMDC after transfection and 5 days culture. Is that too small population?
Thanks for your help!!!
I can only get 6 million BMDC from 1 mouse, and only 1 million BMDC after transfection and 5 days culture. Is that too small population?
Thanks for your help!!!
#3
Astarte Biologics
-
- Active Members
-
- 20 posts
member
2
Neutral
Neutral
Posted 17 November 2012 - 06:19 PM
I get 2-3 times the input cell number after 9 days with GM-CSF/IL4. See this paper and others from this group. An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow.
Lutz MB, Kukutsch N, Ogilvie AL, Rössner S, Koch F, Romani N, Schuler G.
J Immunol Methods. 1999 Feb 1;223(1):77-92.
The lot of fetal calf serum used is important.
Lutz MB, Kukutsch N, Ogilvie AL, Rössner S, Koch F, Romani N, Schuler G.
J Immunol Methods. 1999 Feb 1;223(1):77-92.
The lot of fetal calf serum used is important.
