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TOPO TA Cloning

CLONING TOPO TA BACTERIAL CULTURE SUBCULTURE

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#1 Epigeneticist

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Posted 10 November 2012 - 08:24 PM

I recently used the TOPO TA kit for cloning. After plating my transformed bacteria on LB amp I refrigerated the remaining broth culture. The broth culture consists of the transformed cells that were grown in LB (no amp) for 1-hour. How long is this culture good for when stored at 4C? Do I need to subculture before plating on selective media or can I plate directly to selective media?

#2 phage434

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Posted 10 November 2012 - 09:18 PM

You should be able to directly plate on selective media. I would not store it more than a few days at 4C.





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