I recently used the TOPO TA kit for cloning. After plating my transformed bacteria on LB amp I refrigerated the remaining broth culture. The broth culture consists of the transformed cells that were grown in LB (no amp) for 1-hour. How long is this culture good for when stored at 4C? Do I need to subculture before plating on selective media or can I plate directly to selective media?
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TOPO TA Cloning
Started by Epigeneticist, Nov 10 2012 08:24 PM
CLONING TOPO TA BACTERIAL CULTURE SUBCULTURE
1 reply to this topic
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phage434
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Posted 10 November 2012 - 09:18 PM
You should be able to directly plate on selective media. I would not store it more than a few days at 4C.
Also tagged with one or more of these keywords: CLONING, TOPO TA, BACTERIAL CULTURE, SUBCULTURE
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