Wanna Help, Here's an example I'd use to ask my questions regarding how genes are expressed in a plasmid. In the pBR322 below, I’ve noticed that there are two antibiotic resistance markers going in opposite directions. Also, the origin is going in the counter clockwise direction. Could you please let me know now I'm using tetracycline to select for my transformant, how'd the TetR gene be read since it’s on a collision course with the ori?
Submit your paper to J Biol Methods today!
different orientation or direction of genes in expression Plasmid vector
Started by Akila Wijerathna Yapa, Nov 10 2012 06:19 PM
Vectors Plasmid Coning
4 replies to this topic
#1
Akila Wijerathna Yapa
-
- Active Members
-
- 13 posts
member
0
Neutral
Neutral
Posted 10 November 2012 - 06:19 PM
Dear all,
Wanna Help, Here's an example I'd use to ask my questions regarding how genes are expressed in a plasmid. In the pBR322 below, I’ve noticed that there are two antibiotic resistance markers going in opposite directions. Also, the origin is going in the counter clockwise direction. Could you please let me know now I'm using tetracycline to select for my transformant, how'd the TetR gene be read since it’s on a collision course with the ori?
Wanna Help, Here's an example I'd use to ask my questions regarding how genes are expressed in a plasmid. In the pBR322 below, I’ve noticed that there are two antibiotic resistance markers going in opposite directions. Also, the origin is going in the counter clockwise direction. Could you please let me know now I'm using tetracycline to select for my transformant, how'd the TetR gene be read since it’s on a collision course with the ori?
#2
bob1
-
- Global Moderators
-
- 5,521 posts
Thelymitra pulchella
371
Excellent
Excellent
Posted 10 November 2012 - 07:56 PM
As I think this is a homework question which you should be thinking about yourself - think about the function of each of the parts you are looking at - the ori is the origin of replication, Tetr is a gene....
- Akila Wijerathna Yapa likes this
#3
Julio-Claudian
-
- Active Members
-
- 78 posts
i'm unmethylated
6
Neutral
Neutral
#4
Akila Wijerathna Yapa
-
- Active Members
-
- 13 posts
member
0
Neutral
Neutral
Posted 11 November 2012 - 04:13 AM
Dear
Dear bob1;
Thank you
As I think this is a homework question which you should be thinking about yourself - think about the function of each of the parts you are looking at - the ori is the origin of replication, Tetr is a gene....
Dear bob1;
Thank you
#5
Akila Wijerathna Yapa
-
- Active Members
-
- 13 posts
member
0
Neutral
Neutral
Posted 11 November 2012 - 04:15 AM
The answers to your 'question'
can be found here http://www.protocol-...s-in-a-plasmid/
Dear Julio-Claudian;
Thank you very much for your kind replyn
Also tagged with one or more of these keywords: Vectors, Plasmid, Coning
 |
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Plasmid calculation question (Standard Curve Quantification)Started by Luiz Fernando Lino, Yesterday, 06:42 AM  Standard Curve Quantific, qPCR and 2 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Biology →
What is the current best site-direct mutagenesis Kits?Started by ionchannelbk, 09 Jul 2014 Mutagenesis, plasmid
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Lentivirus copy numberStarted by Zduan, 08 Jul 2014 lentivirus, experimental design and 3 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Markerless replacement in E. coliStarted by Raygoza, 01 Jul 2014 recombination, plasmid and 1 more...
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Miniprep gives high yield but maxiprep gives no plasmid?Started by Epigeneticist, 22 Jun 2014 Maxiprep, Miniprep, Low yield and 2 more...
|
|
|
