Wanna Help, Here's an example I'd use to ask my questions regarding how genes are expressed in a plasmid. In the pBR322 below, I’ve noticed that there are two antibiotic resistance markers going in opposite directions. Also, the origin is going in the counter clockwise direction. Could you please let me know now I'm using tetracycline to select for my transformant, how'd the TetR gene be read since it’s on a collision course with the ori?
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different orientation or direction of genes in expression Plasmid vector
Started by Akila Wijerathna Yapa, Nov 10 2012 06:19 PM
Vectors Plasmid Coning
4 replies to this topic
#1
Akila Wijerathna Yapa
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Posted 10 November 2012 - 06:19 PM
Dear all,
Wanna Help, Here's an example I'd use to ask my questions regarding how genes are expressed in a plasmid. In the pBR322 below, I’ve noticed that there are two antibiotic resistance markers going in opposite directions. Also, the origin is going in the counter clockwise direction. Could you please let me know now I'm using tetracycline to select for my transformant, how'd the TetR gene be read since it’s on a collision course with the ori?
Wanna Help, Here's an example I'd use to ask my questions regarding how genes are expressed in a plasmid. In the pBR322 below, I’ve noticed that there are two antibiotic resistance markers going in opposite directions. Also, the origin is going in the counter clockwise direction. Could you please let me know now I'm using tetracycline to select for my transformant, how'd the TetR gene be read since it’s on a collision course with the ori?
#2
bob1
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Posted 10 November 2012 - 07:56 PM
As I think this is a homework question which you should be thinking about yourself - think about the function of each of the parts you are looking at - the ori is the origin of replication, Tetr is a gene....
- Akila Wijerathna Yapa likes this
#3
Julio-Claudian
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#4
Akila Wijerathna Yapa
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Posted 11 November 2012 - 04:13 AM
Dear
Dear bob1;
Thank you
As I think this is a homework question which you should be thinking about yourself - think about the function of each of the parts you are looking at - the ori is the origin of replication, Tetr is a gene....
Dear bob1;
Thank you
#5
Akila Wijerathna Yapa
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Posted 11 November 2012 - 04:15 AM
The answers to your 'question'
can be found here http://www.protocol-...s-in-a-plasmid/
Dear Julio-Claudian;
Thank you very much for your kind replyn
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