Wanna Help, Here's an example I'd use to ask my questions regarding how genes are expressed in a plasmid. In the pBR322 below, I’ve noticed that there are two antibiotic resistance markers going in opposite directions. Also, the origin is going in the counter clockwise direction. Could you please let me know now I'm using tetracycline to select for my transformant, how'd the TetR gene be read since it’s on a collision course with the ori?
Submit your paper to J Biol Methods today!
different orientation or direction of genes in expression Plasmid vector
Started by Akila Wijerathna Yapa, Nov 10 2012 06:19 PM
Vectors Plasmid Coning
4 replies to this topic
#1
Akila Wijerathna Yapa
-
- Active Members
-
- 13 posts
member
0
Neutral
Neutral
Posted 10 November 2012 - 06:19 PM
Dear all,
Wanna Help, Here's an example I'd use to ask my questions regarding how genes are expressed in a plasmid. In the pBR322 below, I’ve noticed that there are two antibiotic resistance markers going in opposite directions. Also, the origin is going in the counter clockwise direction. Could you please let me know now I'm using tetracycline to select for my transformant, how'd the TetR gene be read since it’s on a collision course with the ori?
Wanna Help, Here's an example I'd use to ask my questions regarding how genes are expressed in a plasmid. In the pBR322 below, I’ve noticed that there are two antibiotic resistance markers going in opposite directions. Also, the origin is going in the counter clockwise direction. Could you please let me know now I'm using tetracycline to select for my transformant, how'd the TetR gene be read since it’s on a collision course with the ori?
#2
bob1
-
- Global Moderators
-
- 5,240 posts
Thelymitra pulchella
336
Excellent
Excellent
Posted 10 November 2012 - 07:56 PM
As I think this is a homework question which you should be thinking about yourself - think about the function of each of the parts you are looking at - the ori is the origin of replication, Tetr is a gene....
- Akila Wijerathna Yapa likes this
#3
Julio-Claudian
-
- Active Members
-
- 78 posts
i'm unmethylated
6
Neutral
Neutral
#4
Akila Wijerathna Yapa
-
- Active Members
-
- 13 posts
member
0
Neutral
Neutral
Posted 11 November 2012 - 04:13 AM
Dear
Dear bob1;
Thank you
As I think this is a homework question which you should be thinking about yourself - think about the function of each of the parts you are looking at - the ori is the origin of replication, Tetr is a gene....
Dear bob1;
Thank you
#5
Akila Wijerathna Yapa
-
- Active Members
-
- 13 posts
member
0
Neutral
Neutral
Posted 11 November 2012 - 04:15 AM
The answers to your 'question'
can be found here http://www.protocol-...s-in-a-plasmid/
Dear Julio-Claudian;
Thank you very much for your kind replyn
Also tagged with one or more of these keywords: Vectors, Plasmid, Coning
Protocols and Techniques Forums →
Genetics and Genomics →
Can we add a restriction site to a gene?Started by FogtBiotech, 12 Mar 2014  DNA, gene, transformation and 2 more...
|
|
|
||
Protocols and Techniques Forums →
Microbiology →
E. coli growing in LB-ampicillin medium without plasmid? Or miniprep problem?Started by Paperdog, 04 Mar 2014 E. coli, selection, plasmid
|
|
|
||
Protocols and Techniques Forums →
Molecular Cloning →
fluorescent tag on membrane proteinStarted by mengkong2, 26 Feb 2014 fluorescent, cloning, plasmid
|
|
|
||
Protocols and Techniques Forums →
Molecular Cloning →
How to obtain Vectors used in articlesStarted by tihong10, 18 Feb 2014 procuring, vectors
|
|
|
||
Protocols and Techniques Forums →
Molecular Cloning →
How to store a ligation reaction over the weekend?Started by joesep, 23 Jan 2014 Ligation, Vector, Plasmid, DNA
|
|
|
