Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

problem by using percoll to purify primary cell


  • Please log in to reply
1 reply to this topic

#1 huaexe

huaexe

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 02 December 2003 - 05:40 AM

when i do cell isolation, i use 20%/50% percoll to purify cell.
when add the cell suspension to the top of percoll, after centrifuge, the cell seem to clot together, finally the middle layer contains very little cell i wanted, but contain many other contamination cell types.
does anyone work on percoll , i am very apprecaited if someone can give me some comments

#2 anilkumarpr

anilkumarpr

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 05 January 2004 - 02:24 AM

Hi,
Percoll centrifugation will give a good result based on the speed, and the percentage (density) of gradient. Some time shape of the tube also affects.
You are using two step percoll gradient. One is 50% and other is 20%. There may be a factor of dilution when you add cell suspension. You can take care of the exact density of the percoll gradients you use.
Avoid mixing while you load low density on top of 50 %

Dont know what type of cell you are interested in. Anyway this is the basic knowledge I have regarding percoll to share with you

Best Wishes

Anil




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.