How does DEPC work to inactivate RNases
Posted 09 November 2012 - 09:48 AM
Posted 09 November 2012 - 01:04 PM
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 10 November 2012 - 12:01 AM
But the RNAse is "killed" by DEPC, by modifying -NH, -SH, and -OH groups in the proteins. After treatment you usually autoclave the solution and get rid of the remaining DEPC (autoclaving breaks down the molecule) because it should not be there later when you work with RNA ("traces of DEPC modify purine residues (A+G) in RNA by carboxymethylation"; [url="http://openwetware.org/wiki/DEPC"]Openwetware[/url]).
Ah, chemical modification, got it. Thanks.
Posted 11 January 2013 - 04:57 AM