I isolated some corynebacteria from bird. I streaked them on LB+Tween agar several times and there was good growth after 72h of incubation. Then, I sealed the plates with parafilm and stored them at 4°C for 3 months.
Now, I have serious problems in cultivating them. They do not grow on LB+Tween plates. Even in LB+Tween broth there is no obserable growth.
Is there a general method for re-activating these bacteria.
Next time, I will try to grow the bacteri in liquid medium, add 50% glycerol and store them at -80°C. Maybe this helps.
Another question is how long I can store sheep blood at 4°C before adding them to liquid agar?
Thanks a lot!
Edited by czernobill, 08 November 2012 - 07:21 AM.