Posted 08 November 2012 - 04:30 AM
I have some ideas regarding immunocytochemistry.
They are more like questions actually.
Is there a good way to protect samples from bleaching? Samples with a bound flourescent secondary antibody?
I used to use vectashield in another lab but here everyone is using glycerol/PBS. Will there be a diffrence?
Can I add something to make it "vectashield-ish"?
From what I understtod vectashield are cheap chemicals sold expensively...
PS. And do I need to bother at all?
Posted 08 November 2012 - 01:35 PM
Posted 12 November 2012 - 08:08 AM
At any rate I´ll start looking at the company products you mentioned.
Posted 20 November 2012 - 10:31 PM
But as all of them are quite expensive. At least this one (I use it) is a mounting medium rather than just a antifading reagent. It may contain some polymer based on the plastic/silicone-like residue it left on the rim of the bottle.
I don't know about your worms but most fluorescent-stained stuff is recommended to be stored at -20° C rather than 4° C, though the later is common for short term storage: examination the next day or so.