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fMLP for neutrophil recruitment protocol?

immunologyneutrophil fMLP lavage

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#1 ImmunoKR

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Posted 08 November 2012 - 01:34 AM

Hi all,

I am having trouble inducing neutrophil influx to the peritoneal cavity in mice using fMLP. I have tried 10mM and 20mM fMLP concentrations (200ul i.p.) and waiting for 2hr and 4hr post-injection to lavage the peritoneal cavity with 10ml HBSS and obtain cells. I am not seeing an increase in the number of cells migrating to the peritoneal cavity compared to a PBS i.p. control. If you have any ideas regarding why my protocol isn't working, or suggestions to improve it, I would greatly appreciate the help!

Thanks

#2 Astarte Biologics

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Posted 17 November 2012 - 06:27 PM

I don't know why the fMLP isn't working but I can tell you that if you use thioglycollate medium you can get neutrophils. 2 mL medium intraperitoneally, wash out after 4 hours.




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